ForHosting KIT · Developer Utilities

Serial dilution volumes calculator

A serial dilution volumes calculator tells you how many microlitres of sample to transfer and how much diluent to add in every tube so each step multiplies the dilution by a chosen fold.

● BetaFree · in your browser

Run — free How it works & API

Use it from WebAPIEmailTelegramApp soon

Bench scientists, ELISA plate setters, microbiology teaching labs, and assay-prep scripts all need the same identity: sample volume equals tube volume divided by the per-step factor, and diluent volume equals the remainder. A 1:10 series in 1 000 µL tubes is therefore 100 µL of sample plus 900 µL of diluent, repeated down the rack; a 2-fold ELISA curve in 200 µL wells is 100 µL plus 100 µL. This endpoint applies that identity for any finite factor greater than one, any positive tube volume, and one to sixty-four steps, and it lists every tube with the source of the aliquot, the transfer out to the next tube, the leftover volume after that transfer, and the cumulative fold from the stock. Optional stock concentration, when supplied, annotates each tube with C0 divided by factor to the tube index so a lab notebook can print both pipette volumes and expected titres from one call. The same deterministic module powers the free browser runner and the API, so a printed SOP, an LMS homework check, and a plate-prep script never disagree on whether a 10-fold, 1 000 µL, four-step series uses 100 µL of stock and 3 600 µL of diluent in total.

How to use it

Enter your values in the form above. The tool checks them before calculating and shows the result on the same page.

Check your inputs

Use the labels and units shown next to each field. If something is missing or outside the allowed range, the page points to the field to fix.

Use it again or automate it

Use the browser tool for individual checks and the API when you need the same capability in an automated workflow.

What you can do with it

Get an answer now

Enter one set of values and see the result without building a spreadsheet or script.

Compare scenarios

Change one value at a time and rerun the calculation to understand what affects the result.

Automate repeated work

Use the API when the same calculation needs to run inside your product or workflow.

FAQ

How do I use this capability?

Complete the fields above and run it on this page. The form highlights anything that needs attention.

For developers — API access

Everything on this page is available programmatically. This section is for teams who want to wire it into their own systems; everyone else can just use the tool above.

API endpoint

POSThttps://api.kit.forhosting.com/bio/serial-dilution-volumes

Prefer to automate it? One authenticated POST creates the task; the result comes back by webhook or a signed link. The same capability also runs here on the web, by email and from Telegram — and soon from our app too.

Call it from your stack

curl -X POST https://api.kit.forhosting.com/bio/serial-dilution-volumes \
  -H "Authorization: Bearer $KIT_KEY" \
  -H "Content-Type: application/json" \
  -d '{"dilution_factor":10,"tube_volume":1000,"steps":4}'

const res = await fetch("https://api.kit.forhosting.com/bio/serial-dilution-volumes", {
  method: "POST",
  headers: {
    "Authorization": `Bearer ${process.env.KIT_KEY}`,
    "Content-Type": "application/json"
  },
  body: JSON.stringify({
    "dilution_factor": 10,
    "tube_volume": 1000,
    "steps": 4
  })
});
const { task_id } = await res.json();

import os, requests

res = requests.post(
    "https://api.kit.forhosting.com/bio/serial-dilution-volumes",
    headers={"Authorization": f"Bearer {os.environ['KIT_KEY']}"},
    json={
    "dilution_factor": 10,
    "tube_volume": 1000,
    "steps": 4
},
)
task_id = res.json()["task_id"]

<?php
$res = file_get_contents("https://api.kit.forhosting.com/bio/serial-dilution-volumes", false, stream_context_create([
  "http" => [
    "method"  => "POST",
    "header"  => "Authorization: Bearer " . getenv("KIT_KEY") . "\r\nContent-Type: application/json",
    "content" => '{"dilution_factor":10,"tube_volume":1000,"steps":4}',
  ],
]));
$task = json_decode($res, true);

body := bytes.NewBufferString(`{"dilution_factor":10,"tube_volume":1000,"steps":4}`)
req, _ := http.NewRequest("POST", "https://api.kit.forhosting.com/bio/serial-dilution-volumes", body)
req.Header.Set("Authorization", "Bearer "+os.Getenv("KIT_KEY"))
req.Header.Set("Content-Type", "application/json")
res, _ := http.DefaultClient.Do(req)

Example request

{
  "dilution_factor": 10,
  "tube_volume": 1000,
  "steps": 4
}

Example response

{
  "task_id": "tsk_a1b2c3d4e5f6a1b2c3d4e5f6",
  "type": "bio.serial_dilution_volumes",
  "status": "queued",
  "_links": {
    "result": "/tasks/tsk_…/result"
  }
}

The API is asynchronous: the call returns a task_id immediately and the result arrives by webhook. Polling is capped at 1 req/s per task.

Pricing

Per request$0.002

Published price — no tokens, no invented credits. A failed task is never charged.

Limits

`max_steps`	64
`max_volume`	1000000000000000
`max_factor`	1000000000000

Errors

HTTP	Code	Meaning
`401`	`unauthorized`	Missing or invalid API key.
`402`	`insufficient_balance`	Your balance doesn't cover the task price.
`404`	`unknown_type`	That task type doesn't exist.
`429`	`rate_limited`	Too many requests. Use the webhook instead of polling.

Read the full KIT documentation →